All lanes : Anti-IL7R alpha antibody [EPR2955(2)] (ab180521) at 1/20000 dilutionLane 1 : K562 cell lysateLane 2 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
All lanes : Anti-IL7R alpha antibody [EPR2955(2)] (ab180521) at 1/5000 dilutionLane 1 : Jurkat cell lysateLane 2 : Daudi cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Flow cytometric analysis of K562 cells (2% paraformaldehyde-fixed) labeling IL7R alpha with ab180521 at 1/200 dilution (red) compared with a Rabbit monoclonal IgG negative control (green). Goat anti rabbit IgG (FITC) secondary antibody was used at 1/75 dilution.