Anti-ILF2 antibody [EPR10695(B)]

• Supplier: Abcam
• Catalog: ab154791
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR10695(B)
• Applications: WB IHC-P FC IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to residues in Human ILF2 (UniProt Q12905)
• Description: Rabbit Monoclonal
• Gene: ILF2
• Conjugate: Unconjugated

Product images

All lanes : Anti-ILF2 antibody [EPR10695(B)] (ab154791) at 1/1000 dilutionLane 1 : K562 cell lysateLane 2 : Raji cell lysateLane 3 : A431 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling ILF2 with ab154791 at 1/50 dilution.

Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling ILF2 with ab154791 at 1/50 dilution.

Immunohistochemical analysis of paraffin embedded Human glioma tissue using ab154791 showing +ve staining.

Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using ab154791 showing +ve staining.

Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154791 showing +ve staining.

Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab154791 showing +ve staining.

Overlay histogram showing HeLa cells stained with ab154791 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab154791, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.