Anti-ILKAP antibody [EPR16145] (ab196013) at 1/20000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-ILKAP antibody [EPR16145] (ab196013) at 1/20000 dilution + 293 (Human epithelial cells from embryonic kidney) cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-ILKAP antibody [EPR16145] (ab196013) at 1/5000 dilution + Mouse brain lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-ILKAP antibody [EPR16145] (ab196013) at 1/1000 dilution + Rat brain lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunoprecipitation analysis of 293 (Human epithelial cells from embryonic kidney) whole cell extract labeling ILKAP using ab196013 at 1/30 dilution (Lane 2).Lane 3: IP using Rabbit monoclonal IgG (ab172730) instead of ab196013 in 293 (Human epithelial cells from embryonic kidney) whole cell extract.Lane 1: Input: 10 μg 293 (Human epithelial cells from embryonic kidney) whole cell extract.Subsequent WB detection was performed using ab196013 at 1/1000 dilution.An Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.