Flow cytometric analysis of PC3 cells labeling IMPA1 using ab191570at 1/300 dilution (red), Isotype control (green). Secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. Cells were fixed in 2% paraformaldehyde prior to staining.
Anti-IMPA1 [EPR15405] antibody (ab191570) at 1/20000 dilution + Mouse brain lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-IMPA1 [EPR15405] antibody (ab191570) at 1/20000 dilutionLane 1 : Jurkat cell lysateLane 2 : PC3 cell lysateLane 3 : HCT 116 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-IMPA1 [EPR15405] antibody (ab191570) at 1/100000 dilution + Human testis lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution