Anti-IMPA1 antibody [EPR15404(B)]

• Supplier: Abcam
• Catalog: ab184165
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR15404(B)
• Applications: FC WB IP ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human IMPA1 aa 50-150
• Description: Rabbit Monoclonal
• Gene: IMPA1
• Conjugate: Unconjugated

Product images

All lanes : Anti-IMPA1 antibody [EPR15404(B)] (ab184165) at 1/50000 dilutionLane 1 : Human testis tissue lysateLane 2 : Jurkat cell line lysateLane 3 : PC 3 cell line lysateLane 4 : HCT116 cell line lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Immunofluorescent analysis of HCT116 cells (4% paraformaldehyde-fixed) labeling IMPA1 with ab184165 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Western blot analysis of immunoprecipitation pellet from Human testis tissue lysate (lane 1) or a PBS control (lane 2) immunoprecipitated using ab184165 at 1/50 dilution.Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. 

Flow cytometric analysis of PC3 cells (2% paraformaldehyde-fixed) labeling IMPA1 with ab184165 at 1/40 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.