Anti-Indoleamine 2, 3-dioxygenase antibody
| Name | Anti-Indoleamine 2, 3-dioxygenase antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab55305 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Applications | IHC-F WB IHC-P FC |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 1-404 of Human Indoleamine 2, 3-dioxygenase |
| Description | Mouse Monoclonal |
| Gene | IDO1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Indoleamine 2 3-dioxygenase antibody (ab55305) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human spleen.
![Overlay histogram showing HeLa cells stained with ab55305 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55305, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/8472_Indoleamine-2-3-dioxygenase-Primary-antibodies-ab55305-1.jpg)
Overlay histogram showing HeLa cells stained with ab55305 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55305, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Indoleamine-2,3-dioxygenase elevated in tumor-initiating cells is suppressed by - Indoleamine-2,3-dioxygenase elevated in tumor-initiating cells is suppressed by
Stapelberg M, Zobalova R, Nguyen MN, Walker T, Stantic M, Goodwin J, Pasdar EA, Thai T, Prokopova K, Yan B, Hall S, de Pennington N, Thomas SR, Grant G, Stursa J, Bajzikova M, Meedeniya AC, Truksa J, Ralph SJ, Ansorge O, Dong LF, Neuzil J. Free Radic Biol Med. 2014 Feb;67:41-50.
Blunted epidermal L-tryptophan metabolism in vitiligo affects immune response and - Blunted epidermal L-tryptophan metabolism in vitiligo affects immune response and
Schallreuter KU, Salem MA, Gibbons NC, Maitland DJ, Marsch E, Elwary SM, Healey AR. FASEB J. 2012 Jun;26(6):2471-85.
Inhibition of IDO1 suppresses cyclooxygenase-2 and matrix metalloproteinase-9 - Inhibition of IDO1 suppresses cyclooxygenase-2 and matrix metalloproteinase-9
Mei J, Jin LP, Ding D, Li MQ, Li DJ, Zhu XY. Mol Hum Reprod. 2012 Oct;18(10):467-76. Epub 2012 May 24.