ATF-2 (N-96): sc-6233. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human tonsil showing nuclear localization.
Western blot analysis of human recombinant ATF-2 untreated fusion protein (A,C) or phosphorylated by human recombinant JNK1 (B,D). Antibodies tested include ATF-2 (N-96): sc-6233 (A,B) and p-ATF-2 (Thr 71)-R: sc-7982-R (C,D).
ATF-2 (N-96): sc-6233. Western blot analysis of ATF-2 expression in K-562 (A) and A-673 (B) nuclear extracts.
ATF-2 siRNA (h): sc-29205. Western blot analysis of ATF-2 expression in non-transfected control (A) and ATF-2 siRNA transfected (B) HeLa cells. Blot probed with ATF-2 (N-96): sc-6233. Lamin A/C (H-110): sc-20681 used as specificity and loading control.
ChIP analysis of transcription factor binding to the Interferon β promoter before (A) and six hours after (B) Sendai virus infection of HeLa cells. Antibodies tested included NFκB p65 (A): sc-109, ATF-2 (N-96): sc-6233, GCN5 (N-18): sc-6303, Pol II (H-224): sc-9001, TFIID (TBP)(SI-1): sc-273 and CBP (A-22): sc-369. Data kindly provided by G. Mosialos.
ATF-2 (N-96): sc-6233. Western blot analysis of ATF-2 expression in HeLa whole cell lysate (A) and A-673 (B) nuclear extract.
Western blot analysis of ATF-2 phosphorlation in untreated (A,C) and anisomycin induced (B,D) NIH/3T3 cells. Antibodies tested include p-ATF-2 (F-1): sc-8398 (A,B) and ATF-2 (N-96): sc-6233 (C,D).
ATF-2 (N-96): sc-6233. Western blot analysis of ATF-2 expression in non-transfected 293T: sc-117752 (A), mouse ATF-2 transfected 293T: sc-126459 (B) and HeLa (C) whole cell lysates.