Anti-Inhibin alpha antibody - N-terminal (ab175539) at 1/1000 dilution + Mouse testis tissue lysates at 35 µg
All lanes : Anti-Inhibin alpha antibody - N-terminal (ab175539) at 1/1000 dilutionLane 1 : Inhibin alpha 293 cell lysate(non transfected)Lane 2 : Inhibin alpha 293 cell lysate (transiently transfected)Lysates/proteins at 2 µg per lane.
Immunofluorescent analysis of Hela cells labeling Inhibin alpha with ab175539 at 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Immunohistochemical analysis of formalin fixed and paraffin embedded Human testis tissue labeling Inhibin alpha with ab175539 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram) labeling Inhibin alpha with ab175539 at 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.