p-ATF-2 (Thr 71)-R: sc-7982-R. Western blot analysis of ATF-2 phosphorylation in anisomycin-treated NIH/3T3 cells (A-C). Antibody was preincubated with either the cognate phosphopeptide (B) or unphosphorylated peptide (C).
p-ATF-2 (Thr 71)-R: sc-7982-R. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear localization.
p-ATF-2 (Thr 71)-R: sc-7982-R. Immunofluorescence staining of methanol-fixed, anisomycin-treated NIH/3T3 cells showing nuclear staining.
Western blot analysis of human recombinant ATF-2 untreated fusion protein (A,C) or phosphorylated by human recombinant JNK1 (B,D). Antibodies tested include ATF-2 (N-96): sc-6233 (A,B) and p-ATF-2 (Thr 71)-R: sc-7982-R (C,D).
p-ATF-2 (Thr 71)-R: sc-7982-R. Immunoperoxidase staining of formalin fixed, paraffin-embedded human hippocampus tissue showing nuclear staining of neuronal cells and glial cells.
p-ATF-2 (Thr 71): sc-7982. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing nuclear staining of Purkinje cells and subset of cells in granular layer.