All lanes : Anti-Integrin alpha 3 antibody (ab131055)Lane 1 : Rat Heart Tissue LysateLane 2 : HeLa Cell Lysate
Immunohistochemical analysis of parafin embedded Rat brain tissue labelling Integrin alpha 3 with ab131055 at 1μg/ml.
Immunohistochemical analysis of parafin embedded Human intestinal cancer tissue labelling Integrin alpha 3 with ab131055 at 1μg/ml.
ab131055 stained SV40LT-SMC cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab131055 at 5µg/ml overnight at +4°C. The secondary antibody (green) was a goat anti-rabbit DyLight® 488 (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.