Anti-Integrin alpha 3 antibody [J143]
| Name | Anti-Integrin alpha 3 antibody [J143] |
|---|---|
| Supplier | Abcam |
| Catalog | ab11767 |
| Prices | $378.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | J143 |
| Applications | IHC-F ICC/IF |
| Species Reactivities | Human |
| Antigen | 253J bladder cancer cell line |
| Description | Mouse Monoclonal |
| Gene | ITGA3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab11767 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11767, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Signaling events mediated by alpha3beta1 integrin are essential for mammary - Signaling events mediated by alpha3beta1 integrin are essential for mammary
Cagnet S, Faraldo MM, Kreft M, Sonnenberg A, Raymond K, Glukhova MA. Oncogene. 2014 Aug 21;33(34):4286-95.
Novel image analysis approach quantifies morphological characteristics of 3D - Novel image analysis approach quantifies morphological characteristics of 3D
McKeen Polizzotti L, Oztan B, Bjornsson CS, Shubert KR, Yener B, Plopper GE. J Biomed Biotechnol. 2012;2012:102036.