Anti-Integrin beta 3 antibody
| Name | Anti-Integrin beta 3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab131056 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Rat, Human, Mouse |
| Antigen | Synthetic peptide corresponding to C-terminal amino acids of Human Integrin beta 3 |
| Description | Rabbit Polyclonal |
| Gene | ITGB3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab131056 stained HCT116 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum/ 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab131056 at 5 µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488Dylight 488 goat anti-rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-Integrin beta 3 antibody (ab131056) at 0.5 µg/mlLane 1 : Jurkat cell lysateLane 2 : Raji cell lysateLane 3 : CEM cell lysateLane 4 : COLO320 cell lysate