Anti-Integrin linked ILK antibody [EP1593Y]

• Supplier: Abcam
• Catalog: ab52480
• Prices: $392.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1593Y
• Applications: WB IP ICC/IF FC IHC-P
• Species Reactivities: Mouse, Rat, Bovine, Human, Monkey
• Antigen: Synthetic peptide corresponding to residues near N terminus of human Integrin linked ILK
• Description: Rabbit Monoclonal
• Gene: ILK
• Conjugate: Unconjugated

Product images

Overlay histogram showing HEK293 cells stained with ab52480 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52480, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit Alexa Fluor® 488 (IgG; H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) at 1/2000 dilution + COS-1 cell lysate at 10 µgSecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution

Immunohistochemical analysis of paraffin-embedded human heart muscle using ab52480 at a 1/50 dilution.Immunohistochemical analysis of paraffin-embedded human heart muscle using ab52480 at a 1/50 dilution.

All lanes : Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) at 1/2000 dilutionLane 1 : Rat BrainLane 2 : Rat HeartLane 3 : Rat KidneyLane 4 : Rat Spleen

Anti-Integrin linked ILK antibody [EP1593Y] (ab52480) + PC12

Product References

Interference with the contractile machinery of the fibroblastic chondrocyte - Interference with the contractile machinery of the fibroblastic chondrocyte

Rottmar M, Mhanna R, Guimond-Lischer S, Vogel V, Zenobi-Wong M, Maniura-Weber K. Exp Cell Res. 2014 Jan 15;320(2):175-87.

Role of PINCH and its partner tumor suppressor Rsu-1 in regulating liver size and - Role of PINCH and its partner tumor suppressor Rsu-1 in regulating liver size and

Donthamsetty S, Bhave VS, Mars WM, Bowen WC, Orr A, Haynes MM, Wu C, Michalopoulos GK. PLoS One. 2013 Sep 18;8(9):e74625.

Multiple roles of integrin-linked kinase in epidermal development, maturation and - Multiple roles of integrin-linked kinase in epidermal development, maturation and

Judah D, Rudkouskaya A, Wilson R, Carter DE, Dagnino L. PLoS One. 2012;7(5):e36704.

Integrin alphavbeta3 upregulates integrin-linked kinase expression in human - Integrin alphavbeta3 upregulates integrin-linked kinase expression in human

Lossner D, Abou-Ajram C, Benge A, Aumercier M, Schmitt M, Reuning U. J Cell Physiol. 2009 Aug;220(2):367-75.