Anti-Integrin linked ILK antibody [EPR1592]

• Supplier: Abcam
• Catalog: ab76468
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR1592
• Applications: WB FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: A synthetic peptide corresponding to an internal region of human Integrin linked ILK
• Description: Rabbit Monoclonal
• Gene: ILK
• Conjugate: Unconjugated

Product images

All lanes : Anti-Integrin linked ILK antibody [EPR1592] (ab76468) at 1/50000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLane 3 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

ab76468 at 1/100 dilution staining Integrin linked ILK in HeLa cells by Immunofluorescence.

ab76468 at 1/100 dilution staining Integrin linked ILK in HeLa cells by Immunofluorescence.

Overlay histogram showing HEK293 cells stained with ab76468 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76468, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was a goat anti-rabbit DyLight® 488 (IgG; H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.