Anti-intestinal alkaline phosphatase antibody
| Name | Anti-intestinal alkaline phosphatase antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab7322 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | Conjugation DB ELISA IHC-F IP WB IHC-P |
| Species Reactivities | Rabbit, Human |
| Antigen | Full length native protein (purified) corresponding to Human intestinal alkaline phosphatase |
| Description | Rabbit Polyclonal |
| Gene | ALPI |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Human intestinal alkaline phosphatase was immunoprecipitated using 0.5mg SW480 whole cell extract, µg of Rabbit polyclonal to Human intestinal alkaline phosphatase and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, SW480 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab7322.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 60kDa; Human intestinal alkaline phosphatase](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/9475_ab7322-212898-IPVI005ab73228mMod.jpg)
Human intestinal alkaline phosphatase was immunoprecipitated using 0.5mg SW480 whole cell extract, µg of Rabbit polyclonal to Human intestinal alkaline phosphatase and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, SW480 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab7322.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 60kDa; Human intestinal alkaline phosphatase
![Anti-intestinal alkaline phosphatase antibody (ab7322) at 1/10000 dilution + Alkaline Phosphatase (Human Intestine)SecondaryHRP Goat-a-Rabbit IgG [H&L] at 1/4000 dilutionPerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/9476_Ab7322_2.jpg)
Anti-intestinal alkaline phosphatase antibody (ab7322) at 1/10000 dilution + Alkaline Phosphatase (Human Intestine)SecondaryHRP Goat-a-Rabbit IgG [H&L] at 1/4000 dilutionPerformed under reducing conditions.
ab7322 (2µg/ml) staining human intestinal alkaline phosphatase in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of mucosal epithelium.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Product References
Intestinal alkaline phosphatase in the colonic mucosa of children with - Intestinal alkaline phosphatase in the colonic mucosa of children with
Molnar K, Vannay A, Szebeni B, Banki NF, Sziksz E, Cseh A, Gyorffy H, Lakatos PL, Papp M, Arato A, Veres G. World J Gastroenterol. 2012 Jul 7;18(25):3254-9.
Resolvin E1-induced intestinal alkaline phosphatase promotes resolution of - Resolvin E1-induced intestinal alkaline phosphatase promotes resolution of
Campbell EL, MacManus CF, Kominsky DJ, Keely S, Glover LE, Bowers BE, Scully M, Bruyninckx WJ, Colgan SP. Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14298-303. doi:
Incomplete penetrance and phenotypic variability characterize Gdf6-attributable - Incomplete penetrance and phenotypic variability characterize Gdf6-attributable
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Perletti G, Marras E, Dondi D, Osti D, Congiu T, Ferrarese R, de Eguileor M, Tashjian AH Jr. Int J Cancer. 2005 Jan 1;113(1):42-53.
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Gobeil F Jr, Bernier SG, Vazquez-Tello A, Brault S, Beauchamp MH, Quiniou C, Marrache AM, Checchin D, Sennlaub F, Hou X, Nader M, Bkaily G, Ribeiro-da-Silva A, Goetzl EJ, Chemtob S. J Biol Chem. 2003 Oct 3;278(40):38875-83. Epub 2003 Jul 7.