Anti-Intrinsic Factor antibody (ab171418) at 1/100 dilution + K562 cell line lysate at 35 µg
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human stomach tissue labeling Intrinsic Factor with ab171418 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Immunofluorescent analysis of 293 cells labeling Intrinsic Factor with ab171418 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) and DAPI staining (blue).
Flow Cytometric analysis of K562 cells labeling Intrinsic Factor with ab171418 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.