Anti-IQGAP3 antibody (ab88353) at 1 µg/ml + Human placenta lysate at 25 µgSecondaryGoat anti-Mouse IgG at 1/5000 dilution
All lanes : Anti-IQGAP3 antibody (ab88353) at 1 µg/mlLane 1 : IQGAP3-transfected 293T cell lysateLane 2 : Untransfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat anti-Mouse IgG at 1/5000 dilution
All lanes : Anti-IQGAP3 antibody (ab88353) at 1/500 dilutionLane 1 : Mouse lung whole tissue lysateLane 2 : Mouse lung whole tissue lysateLane 3 : Mouse lung adenocarcinoma whole tissue lysateLane 4 : Mouse lung adenocarcinoma whole tissue lysateLysates/proteins at 20 µg per lane.SecondaryHRP-conjugated rabbit anti-mouse IgG monoclonal at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.