All lanes : Anti-IRAK2 antibody [EPR16530] (ab192249) at 1/10000 dilutionLane 1 : K562 cell lysateLane 2 : MOLT4 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow cytometric analysis of 2% paraformaldehyde-fixed MOLT4 cells labeling IRAK2 with ab192249 at 1/220 dilution (pink), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
All lanes : Anti-IRAK2 antibody [EPR16530] (ab192249) at 1/1000 dilutionLane 1 : THP1 cell lysateLane 2 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Western blot analysis of IRAK2 in MOLT4 cell lysates immunoprecipitated using ab192249 at 1/60 dilution. Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.Blocking/Dilution buffer: 5% NFDM /TBST.