Anti-IRF3 antibody
| Name | Anti-IRF3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab25950 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF ELISA WB |
| Species Reactivities | Mouse, Human |
| Antigen | A synthetic peptide corresponding to 14 amino acids near the carboxyterminus of IRF3 (Human) |
| Description | Rabbit Polyclonal |
| Gene | IRF3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab25950 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab25950 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of IRF3 in Mouse Kidney cells using ab25950 at 20 ug/ml.
Lane 1 : Anti-IRF3 antibody (ab25950) at 1 µg/mlLane 2 : Anti-IRF3 antibody (ab25950) at 2 µg/mlLane 3 : Anti-IRF3 antibody (ab25950) at 4 µg/mlLane 1 : Ramos whole cell lysate.Lane 2 : Ramos whole cell lysate.Lane 3 : Ramos whole cell lysate.
ab25950 at 2µg/ml staining IRF3 in mouse kidney by IHC.
Product References
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