Anti-IRF3 antibody [EPR2418Y]

• Supplier: Abcam
• Catalog: ab68481
• Prices: $388.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR2418Y
• Applications: ICC/IF ICC/IF WB IHC-P FC
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human IRF3 aa 50-150
• Blocking Peptide: IRF3 peptide
• Description: Rabbit Monoclonal
• Gene: IRF3
• Conjugate: Unconjugated

Product images

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling IRF3 with ab68481 at 1/100 dilution. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/400 dilution was used as the secondary antibody (green). The confocal image shows cytoplasmic on HeLa cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows;1. ab68481 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution. 

All lanes : Anti-IRF3 antibody [EPR2418Y] (ab68481) at 1/1000 dilutionLane 1 : Human fetal heart lysateLane 2 : Human fetal kidney lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-IRF3 antibody [EPR2418Y] (ab68481) at 1/10000 dilutionLane 1 : THP-1 (Human monocytic leukemia cells) whole cell lysatesLane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysatesLane 3 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-IRF3 antibody [EPR2418Y] (ab68481) at 1/10000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysatesLane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysatesLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-IRF3 antibody [EPR2418Y] (ab68481) at 1/1000 dilutionLane 1 : Mouse heart lysateLane 2 : Mouse spleen lysateLane 3 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysatesLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Flow cytometry analysis of 2% paraformaldehyde fixed U937 (Human histiocytic lymphoma cells) cells labeling IRF3 with ab68481 at 1/160 dilution (red line). Secondary antibody used is a goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is rabbit monoclonal IgG (black line). The unlabeled control is cells without incubation with primary and secondary antibodies (blue line).

Immunohistochemical analysis of paraffin-embedded Human tonsil labeling IRF3 with ab68481at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.

Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix labeling IRF3 with ab68481at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.

Immunohistochemical analysis of paraffin-embedded Mouse spleen labeling IRF3 with ab68481at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. The negative control utilised PBS instead of primary antibody. Counter stained with Hematoxylin.

Product References

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Liu F, Wu S, Ren H, Gu J. Nat Cell Biol. 2011 Mar;13(3):254-62.