Anti-IRF5 antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab2932
• Prices: $401.00
• Sizes: 100 µg
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P ChIP WB IP
• Species Reactivities: Dog, Human, Mouse, Rat, Bovine
• Antigen: Synthetic peptide: QGPWPMHPAGMQ, corresponding to C terminal amino acids 493-504 of Human IRF5
• Description: Goat Polyclonal
• Gene: IRF5
• Conjugate: Unconjugated

Product images

IHC image of ab2932 staining in human normal cervix (ab29226) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2932, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

IRF5 is a target of BCR-ABL kinase activity and reduces CML cell proliferation. - IRF5 is a target of BCR-ABL kinase activity and reduces CML cell proliferation.

Massimino M, Consoli ML, Mesuraca M, Stagno F, Tirro E, Stella S, Pennisi MS, Romano C, Buffa P, Bond HM, Morrone G, Sciacca L, Di Raimondo F, Manzella L, Vigneri P. Carcinogenesis. 2014 May;35(5):1132-43.

Intracellular antibody-bound pathogens stimulate immune signaling via the Fc - Intracellular antibody-bound pathogens stimulate immune signaling via the Fc

McEwan WA, Tam JC, Watkinson RE, Bidgood SR, Mallery DL, James LC. Nat Immunol. 2013 Apr;14(4):327-36.

Cross-interference of RLR and TLR signaling pathways modulates antibacterial T - Cross-interference of RLR and TLR signaling pathways modulates antibacterial T

Negishi H, Yanai H, Nakajima A, Koshiba R, Atarashi K, Matsuda A, Matsuki K, Miki S, Doi T, Aderem A, Nishio J, Smale ST, Honda K, Taniguchi T. Nat Immunol. 2012 May 20;13(7):659-66.

IRF5 promotes inflammatory macrophage polarization and TH1-TH17 responses. - IRF5 promotes inflammatory macrophage polarization and TH1-TH17 responses.

Krausgruber T, Blazek K, Smallie T, Alzabin S, Lockstone H, Sahgal N, Hussell T, Feldmann M, Udalova IA. Nat Immunol. 2011 Mar;12(3):231-8.

IRF5 is required for late-phase TNF secretion by human dendritic cells. - IRF5 is required for late-phase TNF secretion by human dendritic cells.

Krausgruber T, Saliba D, Ryzhakov G, Lanfrancotti A, Blazek K, Udalova IA. Blood. 2010 Jun 3;115(22):4421-30.

Differential requirement of histone acetylase and deacetylase activities for - Differential requirement of histone acetylase and deacetylase activities for

Feng D, Sangster-Guity N, Stone R, Korczeniewska J, Mancl ME, Fitzgerald-Bocarsly P, Barnes BJ. J Immunol. 2010 Nov 15;185(10):6003-12.

IRF-5 is a mediator of the death receptor-induced apoptotic signaling pathway. - IRF-5 is a mediator of the death receptor-induced apoptotic signaling pathway.

Hu G, Barnes BJ. J Biol Chem. 2009 Jan 30;284(5):2767-77.

Methamphetamine enhances Hepatitis C virus replication in human hepatocytes. - Methamphetamine enhances Hepatitis C virus replication in human hepatocytes.

Ye L, Peng JS, Wang X, Wang YJ, Luo GX, Ho WZ. J Viral Hepat. 2008 Apr;15(4):261-70.

Three functional variants of IFN regulatory factor 5 (IRF5) define risk and - Three functional variants of IFN regulatory factor 5 (IRF5) define risk and

Graham RR, Kyogoku C, Sigurdsson S, Vlasova IA, Davies LR, Baechler EC, Plenge RM, Koeuth T, Ortmann WA, Hom G, Bauer JW, Gillett C, Burtt N, Cunninghame Graham DS, Onofrio R, Petri M, Gunnarsson I, Svenungsson E, Ronnblom L, Nordmark G, Gregersen PK, Moser K, Gaffney PM, Criswell LA, Vyse TJ, Syvanen AC, Bohjanen PR, Daly MJ, Behrens TW, Altshuler D. Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6758-63. Epub 2007 Apr 5.

Interferon regulatory factor 5 represses expression of the Epstein-Barr virus - Interferon regulatory factor 5 represses expression of the Epstein-Barr virus

Ning S, Huye LE, Pagano JS. J Virol. 2005 Sep;79(18):11671-6.