Anti-IRS2 (phospho S731) antibody
| Name | Anti-IRS2 (phospho S731) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab3690 |
| Prices | $390.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP ELISA IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide (YKASSpPAESS), corresponding to amino acids 727-736 of human IRS2 at the phosphorylated site of Serine 731 |
| Description | Rabbit Polyclonal |
| Gene | IRS2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IP: 3T3 cell lysate immunoprecipitated with Anti-IRS-2 (pSER731) (ab3690)
ab3690 (2µg/ml) staining IRS2 (phospho S731) in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cell membrane.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Anti-IRS2 (phospho S731) antibody (ab3690) at 1 µg/ml + Brain (Mouse) Tissue Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 160 kDa (why is the actual band size different from the predicted?)Additional bands at : 121 kDa,64 kDa. We are unsure as to the identity of these extra bands.Exposure time : 8 minutes
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