ab52606 stained SKNSH cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52606 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
![Anti-IRS2 antibody [EP976Y] (ab52606) at 1/100000 dilution + 293 cell lysate at 10 µgSecondaryHRP-labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/10219_ab52606wb.jpg)
Anti-IRS2 antibody [EP976Y] (ab52606) at 1/100000 dilution + 293 cell lysate at 10 µgSecondaryHRP-labelled goat anti-rabbit at 1/2000 dilution
Immunohistochemical staining of paraffin-embedded human kidney using ab52606 at a dilution of 1/100-1/250.Immunohistochemical staining of paraffin-embedded human kidney using ab52606 at a dilution of 1/100-1/250.