All lanes : Anti-IRX5 antibody (ab100939) at 1/250 dilutionLane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell LysateLane 4 : DU 145 (Human prostate carcinoma cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.