Anti-Isocitrate dehydrogenase antibody

• Supplier: Abcam
• Catalog: ab154932
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB IP IHC-P ICC/IF ICC/IF
• Species Reactivities: Mouse, Human
• Antigen: Recombinant fragment, corresponding to a region within amino acids 1-190 of Human Isocitrate dehydrogenase (Uniprot ID: O75874)
• Description: Rabbit Polyclonal
• Gene: IDH1
• Conjugate: Unconjugated

Product images

All lanes : Anti-Isocitrate dehydrogenase antibody (ab154932) at 1/5000 dilutionLane 1 : A431 whole cell lysate/extractLane 2 : HepG2 whole cell lysate/extractLysates/proteins at 30 µg per lane.

All lanes : Anti-Isocitrate dehydrogenase antibody (ab154932) at 1/10000 dilutionLane 1 : Neuro2A whole cell lysateLane 2 : GL261 whole cell lysateLane 3 : C8D30 whole cell lysateLane 4 : NIH 3T3 whole cell lysateLane 5 : Raw 264.7 whole cell lysateLane 6 : C2C12 whole cell lysateLysates/proteins at 30 µg per lane.

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Isocitrate dehydrogenase with ab154932 at 1/500.

Immunofluorescent analysis of methanol-fixed A431 cells labeling Isocitrate dehydrogenase using ab154932 at 1/500 (upper image) and costained with Hoechst 33342 (lower image).

Immuprecipitation of Isocitrate dehydrogenase protein in 1000 µg HeLa whole cell lysate/extract using: 1) 40 µg HeLa whole cell lysate/extract, 2) Control with 2.5 µg of preimmune Rabbit IgG and 3) 2.5 µg of ab154932. The immunoprecipitated protein was detected by ab154932 at 1/1000 using Western Blot analysis. Anti Rabbit IgG was used as a secondary antibody. 12% SDS-PAGE.