Anti-Isocitrate dehydrogenase antibody [GT1521]

• Supplier: Abcam
• Catalog: ab184615
• Prices: $384.00
• Sizes: 100 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone: GT1521
• Applications: ICC/IF ICC/IF IP WB
• Species Reactivities: Mouse, Rat, Human, Monkey
• Antigen: Recombinant fragment within Human Isocitrate dehydrogenase aa 30-337
• Description: Mouse Monoclonal
• Gene: IDH1
• Conjugate: Unconjugated

Product images

Immunoprecipitaion analysis:Lane1: 30 μg whole cell lysate/extract of Isocitrate dehydrogenase expressing HepG2 cells;Lane 2: Control with 2.5 μg of pre-immune mouse IgG;Lane 3: Immunoprecipitation of Isocitrate dehydrogenase protein by 2.5 μg of ab184615. The immunoprecipitated Isocitrate dehydrogenase protein was detected by western blot using ab184615 diluted at 1 : 1000.

Immunofluorescent analysis of ice-cold MeOH fixed (for 5 min) A431 cells labeling Isocitrate dehydrogenase at a 1/200 dilution (green), with Hoechst 33342 counterstaining (blue).

All lanes : Anti-Isocitrate dehydrogenase antibody [GT1521] (ab184615) at 1/1000 dilutionLane 1 : Neuro2A whole cell lysate/extractLane 2 : GL261 whole cell lysate/extractLane 3 : C8D30 whole cell lysate/extractLane 4 : NIH-3T3 whole cell lysate/extractLane 5 : Raw264.7 whole cell lysate/extractLane 6 : C2C12 whole cell lysate/extractLysates/proteins at 30 µg per lane.

All lanes : Anti-Isocitrate dehydrogenase antibody [GT1521] (ab184615) at 1/1000 dilutionLane 1 : PC-12 whole cell lysate/extractLane 2 : Rat-2 whole cell lysate/extractLysates/proteins at 30 µg per lane.

All lanes : Anti-Isocitrate dehydrogenase antibody [GT1521] (ab184615) at 1/1000 dilutionLane 1 : 293T whole cell lysate/extractLane 2 : A431 whole cell lysate/extractLane 3 : HeLa whole cell lysate/extractLane 4 : HepG2 whole cell lysate/extractLysates/proteins at 30 µg per lane.