Anti-Isoleucyl tRNA synthetase antibody
| Name | Anti-Isoleucyl tRNA synthetase antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab31533 |
| Prices | $370.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | S1074-F1264 denatured recombinant human IRS |
| Description | Rabbit Polyclonal |
| Gene | IARS |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab31533 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31533, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-Isoleucyl tRNA synthetase antibody (ab31533) at 1/2000 dilution + whole cell lysate prepared from HEK293 cells at 50 µg/mlSecondaryGoat anti-rabbit IgG (H+L) at 1/10000 dilution