ICC/IF (Immunocytochemistry/immunofluorescence) of Human cell line U-2 OS labelling ISY1 (green) with ab121250 at 1µg/ml. Cells were fixed with PFA and permeabilized with Triton X-100. Immunofluorescent staining shows positivity in nucleus but not nucleoli.
All lanes : Anti-ISY1 antibody (ab121250) at 1/250 dilutionLane 1 : Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2 Lane 2 : RT-4 cell lysateLane 3 : U-251 MG cell lysateLane 4 : Human plasma lysateLane 5 : Liver lysateLane 6 : Tonsil lysate
Immunohistochemical staining of paraffin embedded Human stomach, using 1/75 ab121250, shows moderate nuclear positivity in glandular cells.
Immunohistochemical staining of Human stomach shows moderate nuclear positivity in glandular cells using ab121250