Immunohistochemical analysis of paraffin-embedded Human plasma tissue labeling ITIH4 with ab180139 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.
Western blot analysis of ITIH4 in immunoprecipitation pellets from Human plasma lysate. ab180139 used at 1/60 dilution for immunoprecipitation. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000. Blocking buffer and concentration: 5% NFDM/TBST.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ITIH4 with ab180139 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.
All lanes : Anti-ITIH4 antibody [EPR11233(2)] (ab180139) at 1/5000 dilutionLane 1 : Mouse serumLane 2 : Mouse plasmaLane 3 : Rat serumLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1000 µg
Anti-ITIH4 antibody [EPR11233(2)] (ab180139) at 1/5000 dilution + Human plasma at 10 µgSecondaryAnti-Rabbit IgG (HRP) at 1/1000 dilution