Western blot analysis of JAML in 293 cell lysate immunoprecipitated using ab183714 at 1/50 dilution (Lane 1). Lane 2 : TBS instead of 293 lysate.Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution
Flow cytometric analysis of 2% paraformaldehyde-fixed Molt-4 cells labeling JAML with ab183714 at 1/160 dilution (pink) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
All lanes : Anti-JAML antibody [EPR15289] (ab183714) at 1/5000 dilutionLane 1 : C6 cell lysateLane 2 : Raw264.7 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
All lanes : Anti-JAML antibody [EPR15289] (ab183714) at 1/20000 dilutionLane 1 : Human fetal liver lysateLane 2 : Jurkat cell lysateLane 3 : Molt-4 cell lysateLane 4 : 293 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution