Anti-JunB antibody
| Name | Anti-JunB antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab31421 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IP IHC-P ELISA |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic non-phosphopeptide derived from Human JunB around the phosphorylation site of Serine 259 |
| Description | Rabbit Polyclonal |
| Gene | JUNB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab31421 stained Hek293 cells (ab7902). The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31421, 5µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-JunB antibody (ab31421) at 1/500 dilutionLane 1 : Extracts of Hela cells.Lane 2 : Extracts of Hela cells. Antibody pre-incubated with synthesized peptide.
ab31421 at a 1:50-1:100 dilution, staining JunB in human breast carcinoma using Immunohistochemistry, Paraffin Embedded Tissue. Left image : Un-treated. Right image : Antibody pre-incubated with synthesized peptide.
Product References
Interplay between the HTLV-2 Tax and APH-2 proteins in the regulation of the AP-1 - Interplay between the HTLV-2 Tax and APH-2 proteins in the regulation of the AP-1
Marban C, McCabe A, Bukong TN, Hall WW, Sheehy N. Retrovirology. 2012 Dec 3;9:98.
JunB transcription factor maintains skeletal muscle mass and promotes - JunB transcription factor maintains skeletal muscle mass and promotes
Raffaello A, Milan G, Masiero E, Carnio S, Lee D, Lanfranchi G, Goldberg AL, Sandri M. J Cell Biol. 2010 Oct 4;191(1):101-13.
Genetic background influences embryonic lethality and the occurrence of neural - Genetic background influences embryonic lethality and the occurrence of neural
Lemos MC, Harding B, Reed AA, Jeyabalan J, Walls GV, Bowl MR, Sharpe J, Wedden S, Moss JE, Ross A, Davidson D, Thakker RV. J Endocrinol. 2009 Oct;203(1):133-42.
Identifying intrinsic and extrinsic determinants that regulate internal - Identifying intrinsic and extrinsic determinants that regulate internal
Dobson T, Kube E, Timmerman S, Krushel LA. BMC Mol Biol. 2008 Oct 15;9:89.