Anti-JunD antibody [EPR17365]

• Supplier: Abcam
• Catalog: ab181615
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR17365
• Applications: WB IHC-P ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant fragment within Mouse JunD aa 1-150
• Description: Rabbit Monoclonal
• Gene: JUND
• Conjugate: Unconjugated

Product images

All lanes : Anti-JunD antibody [EPR17365] (ab181615) at 1/1000 dilutionLane 1 : 293T (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µgLane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µgLane 3 : Human fetal liver lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Anti-JunD antibody [EPR17365] (ab181615) at 1/1000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-JunD antibody [EPR17365] (ab181615) at 1/1000 dilutionLane 1 : Human fetal brain lysateLane 2 : Human fetal heart lysateLane 3 : Human fetal kidney lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-JunD antibody [EPR17365] (ab181615) at 1/1000 dilutionLane 1 : C6 (Rat glial tumor cells) whole cell lysateLane 2 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus ) whole cell lysateLane 3 : PC12 (Rat adrenal gland pheochromocytoma ) whole cell lysateLane 4 : NIH 3T3 (Mouse embyro fibroblast cells ) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human mammary gland tissue labeling JunD using ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.Inset image: negative control obtained using PBS instead of ab181615 and secondary antibody only.Note: Nuclear staining on the epithelial cells of Human mammary gland was observed.

Immunohistochemical analysis of paraffin-embedded Human lung squamous cell carcinoma tissue labeling JunD using ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.Inset image: negative control obtained using PBS instead of ab181615 and secondary antibody only.Note: Nucleus staining on the cancer cells of lung squamous cell carcinoma was observed.

Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling JunD using ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.Inset image: negative control obtained using PBS instead of ab181615 and secondary antibody only.Note: Nuclear staining on neurons of the mouse cerebral cortex was observed.

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling JunD using ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.Inset image: negative control obtained using PBS instead of ab181615 and secondary antibody only.Note: Nuclear staining on neurons of the rat cerebral cortex was observed.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells, labeling JunD with ab181615 at 1/10000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image shows nuclear staining on the HeLa cell line. The nuclear counter stain is DAPI (blue) . Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:1. ab181615 at 1/10000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.