Anti-Kaiso antibody [6F / 6F8] - ChIP Grade

• Supplier: Abcam
• Catalog: ab12723
• Prices: $403.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 6F / 6F8
• Applications: FC IHC-P ChIP ICC/IF ICC/IF WB IP
• Species Reactivities: Mouse, Rat, Chicken, Hamster, Dog, Human
• Antigen: His-tagged Kaiso fusion protein (Human) lacking the zinc finger region
• Description: Mouse Monoclonal
• Gene: ZBTB33
• Conjugate: Unconjugated

Product images

Immunofluorescence using clone 6F / 6F8 on C2C12 cells.

Overlay histogram showing HeLa cells stained with ab12723 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12723, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Immunofluroesence analysis of Human ARPE-19 cells, staining Kaiso using ab12723.

Product References

Kaiso interacts with p120-catenin to regulate beta-catenin expression at the - Kaiso interacts with p120-catenin to regulate beta-catenin expression at the

Liu Y, Dong QZ, Wang S, Xu HT, Miao Y, Wang L, Wang EH. PLoS One. 2014 Feb 3;9(2):e87537.

KAISO, a critical regulator of p53-mediated transcription of CDKN1A and apoptotic - KAISO, a critical regulator of p53-mediated transcription of CDKN1A and apoptotic

Koh DI, Han D, Ryu H, Choi WI, Jeon BN, Kim MK, Kim Y, Kim JY, Parry L, Clarke AR, Reynolds AB, Hur MW. Proc Natl Acad Sci U S A. 2014 Oct 21;111(42):15078-83. doi:

Kaiso is a key regulator of spleen germinal center formation by repressing Bcl6 - Kaiso is a key regulator of spleen germinal center formation by repressing Bcl6

Koh DI, Yoon JH, Kim MK, An H, Kim MY, Hur MW. Biochem Biophys Res Commun. 2013 Dec 13;442(3-4):177-82. doi:

Selective activation of p120ctn-Kaiso signaling to unlock contact inhibition of - Selective activation of p120ctn-Kaiso signaling to unlock contact inhibition of

Chen HC, Zhu YT, Chen SY, Tseng SC. PLoS One. 2012;7(5):e36864.

Nuclear Kaiso indicates aggressive prostate cancers and promotes migration and - Nuclear Kaiso indicates aggressive prostate cancers and promotes migration and

Jones J, Wang H, Zhou J, Hardy S, Turner T, Austin D, He Q, Wells A, Grizzle WE, Yates C. Am J Pathol. 2012 Nov;181(5):1836-46.

Wnt controls the transcriptional activity of Kaiso through CK1epsilon-dependent - Wnt controls the transcriptional activity of Kaiso through CK1epsilon-dependent

Del Valle-Perez B, Casagolda D, Lugilde E, Valls G, Codina M, Dave N, de Herreros AG, Dunach M. J Cell Sci. 2011 Jul 1;124(Pt 13):2298-309.

Regulation of the rapsyn promoter by kaiso and delta-catenin. - Regulation of the rapsyn promoter by kaiso and delta-catenin.

Rodova M, Kelly KF, VanSaun M, Daniel JM, Werle MJ. Mol Cell Biol. 2004 Aug;24(16):7188-96.

Monoclonal antibodies to Kaiso: a novel transcription factor and p120ctn-binding - Monoclonal antibodies to Kaiso: a novel transcription factor and p120ctn-binding

Daniel JM, Ireton RC, Reynolds AB. Hybridoma. 2001 Jun;20(3):159-66.

The catenin p120(ctn) interacts with Kaiso, a novel BTB/POZ domain zinc finger - The catenin p120(ctn) interacts with Kaiso, a novel BTB/POZ domain zinc finger

Daniel JM, Reynolds AB. Mol Cell Biol. 1999 May;19(5):3614-23.