All lanes : Anti-Kallikrein 10 antibody - Catalytic domain (ab40956) at 1/1000 dilutionLane 1 : Whole cell lysate prepared from DUC-26 human prostate cells (treated with SF) Lane 2 : Whole cell lysate prepared from DUC-26 human prostate cells (treated with TPA) Lane 3 : Whole cell lysate prepared from DUC-26 human prostate cells (treated with IL1-beta) Lane 4 : Whole cell lysate prepared from DUC-26 human prostate cells (treated with TNF-alpha) Lane 5 : Whole cell lysate prepared from DUC-26 human prostate cells (treated with 1 uM testosterone) Lysates/proteins at 5 µl per lane.