All lanes : Anti-Kallikrein 12 antibody - Catalytic domain (ab40958) at 1/1000 dilutionLane 1 : Cell line A2058. SF at 0.01 µgLane 2 : Cell line A2058. TPA at 0.01 µgLane 3 : Cell line A2058. IL1-alpha at 0.01 µgLane 4 : Cell line A2058. TNF-alpha at 0.01 µgLane 5 : Cell line DU 145. SF at 0.01 µgLane 6 : Cell line DU 145. TPA at 0.01 µgLane 7 : Cell line DU 145. IL1-beta at 0.01 µgLane 8 : HFL-1 at 5 µlLane 9 : Cell line LNCaP. SF at 0.01 µgLane 10 : Cell line LNCaP. 0.1uM Testosterone Lane 11 : Cell line A2058. 1.0uM TestosteroneLane 12 : Cell line A2058. 10uM TestosteroneLane 13 : Cell line A2058. TNF-alpha at 0.01 µg