Anti-Kallikrein 5 antibody

• Supplier: Abcam
• Catalog: ab7283
• Prices: $384.00
• Sizes: 50 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB IHC-P
• Species Reactivities: Human
• Antigen: Synthetic peptide, corresponding to amino acids 280-293 of Human KLK5
• Description: Rabbit Polyclonal
• Gene: KLK5
• Conjugate: Unconjugated

Product images

IHC image of ab7283 staining Kallikrein 5 in Human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7283, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

ICC/IF image of ab7283 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7283, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Transgenic kallikrein 5 mice reproduce major cutaneous and systemic hallmarks of - Transgenic kallikrein 5 mice reproduce major cutaneous and systemic hallmarks of

Furio L, de Veer S, Jaillet M, Briot A, Robin A, Deraison C, Hovnanian A. J Exp Med. 2014 Mar 10;211(3):499-513.

Elastase 2 is expressed in human and mouse epidermis and impairs skin barrier - Elastase 2 is expressed in human and mouse epidermis and impairs skin barrier

Bonnart C, Deraison C, Lacroix M, Uchida Y, Besson C, Robin A, Briot A, Gonthier M, Lamant L, Dubus P, Monsarrat B, Hovnanian A. J Clin Invest. 2010 Mar;120(3):871-82.

Self-improvement of keratinocyte differentiation defects during skin maturation - Self-improvement of keratinocyte differentiation defects during skin maturation

Yanagi T, Akiyama M, Nishihara H, Ishikawa J, Sakai K, Miyamura Y, Naoe A, Kitahara T, Tanaka S, Shimizu H. Am J Pathol. 2010 Jul;177(1):106-18.

A heterozygous null mutation combined with the G1258A polymorphism of SPINK5 - A heterozygous null mutation combined with the G1258A polymorphism of SPINK5

Di WL, Hennekam RC, Callard RE, Harper JI. Br J Dermatol. 2009 Aug;161(2):404-12.