All lanes : Anti-Kallikrein 9 antibody - Catalytic domain (ab40955) at 1/1000 dilutionLane 1 : Whole cell lysate prepared from A2058 human melanoma cancer cells (treated with SF) Lane 2 : Whole cell lysate prepared from A2058 human melanoma cancer cells (treated with TPA) Lane 3 : Whole cell lysate prepared from A2058 human melanoma cancer cells (treated with IL1-alpha) Lane 4 : Whole cell lysate prepared from A2058 human melanoma cancer cells (treated with TNF-alpha) Lysates/proteins at 5 µl per lane.