All lanes : Anti-Kallikrein 9 antibody - Kallikrein loop (ab28566) at 1/1000 dilutionLane 1 : Cell line MDA-MB-231. SF at 0.01 µgLane 2 : Cell line MDA-MB-231. 100nM beta-EstradiolLane 3 : Cell line MDA-MB-231. 100nM ProgesteroneLane 4 : Cell line Hs294T. SF at 0.01 µgLane 5 : Cell line Hs294T. TPA at 0.01 µgLane 6 : Cell line Hs294T. IL1-alpha at 0.01 µgLane 7 : Cell line Hs294T. IL1-beta at 0.01 µgLane 8 : Cell line Hs294T. TNF-alpha at 0.01 µgLane 9 : Cell line HT 1080. 10nM beta-EstradiolLane 10 : Cell line HT 1080. 100nM beta-EstradiolLane 11 : Cell line HT 1080. 10nM Progesterone Lane 12 : Cell line HT 1080. 100nM Progesterone