All lanes : Anti-KAT13C / NCOA2 (phospho S736) antibody (ab78899) at 1/500 dilutionLane 1 : extracts from HeLa cells, treated with TSA (400 nM, 24 hours) with no immunizing peptideLane 2 : extracts from HeLa cells, treated with TSA (400 nM, 24 hours) with immunizing peptide at 10 µgLysates/proteins at 10 µg per lane.
Immunohistochemical analysis of KAT13C / NCOA2 in paraffin embedded human breast carcinoma tissue, using a 1/50 dilution of ab78899. Left: untreated sample. Right: sample treated with immunizing peptide.