Anti-KAT13C / NCOA2 antibody - ChIP Grade
| Name | Anti-KAT13C / NCOA2 antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab9261 |
| Prices | $380.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P IP WB ChIP |
| Species Reactivities | Human, Chimpanzee, Ape |
| Antigen | Synthetic peptide (Human) conjugated to KLH - which represents aportion between residues 600 and 700 of the human Nuclear Receptor Coactivator 2 (GenBank PID 1877215) |
| Description | Rabbit Polyclonal |
| Gene | NCOA2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab9261 (2µg/ml) staining KAT13C in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic and nuclear staining.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Product References
Cross talk between glucocorticoid and estrogen receptors occurs at a subset of - Cross talk between glucocorticoid and estrogen receptors occurs at a subset of
Cvoro A, Yuan C, Paruthiyil S, Miller OH, Yamamoto KR, Leitman DC. J Immunol. 2011 Apr 1;186(7):4354-60.
Unliganded estrogen receptor-beta regulation of genes is inhibited by tamoxifen. - Unliganded estrogen receptor-beta regulation of genes is inhibited by tamoxifen.
Levy N, Paruthiyil S, Zhao X, Vivar OI, Saunier EF, Griffin C, Tagliaferri M, Cohen I, Speed TP, Leitman DC. Mol Cell Endocrinol. 2010 Feb 5;315(1-2):201-7.
Selective estrogen receptor-beta agonists repress transcription of - Selective estrogen receptor-beta agonists repress transcription of
Cvoro A, Tatomer D, Tee MK, Zogovic T, Harris HA, Leitman DC. J Immunol. 2008 Jan 1;180(1):630-6.
Differential regulation of native estrogen receptor-regulatory elements by - Differential regulation of native estrogen receptor-regulatory elements by
Levy N, Tatomer D, Herber CB, Zhao X, Tang H, Sargeant T, Ball LJ, Summers J, Speed TP, Leitman DC. Mol Endocrinol. 2008 Feb;22(2):287-303. Epub 2007 Oct 25.
Selective activation of estrogen receptor-beta transcriptional pathways by an - Selective activation of estrogen receptor-beta transcriptional pathways by an
Cvoro A, Paruthiyil S, Jones JO, Tzagarakis-Foster C, Clegg NJ, Tatomer D, Medina RT, Tagliaferri M, Schaufele F, Scanlan TS, Diamond MI, Cohen I, Leitman DC. Endocrinology. 2007 Feb;148(2):538-47. Epub 2006 Nov 9.
Distinct roles of unliganded and liganded estrogen receptors in transcriptional - Distinct roles of unliganded and liganded estrogen receptors in transcriptional
Cvoro A, Tzagarakis-Foster C, Tatomer D, Paruthiyil S, Fox MS, Leitman DC. Mol Cell. 2006 Feb 17;21(4):555-64.