Anti-KAT3A / CBP antibody - ChIP Grade
| Name | Anti-KAT3A / CBP antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab10490 |
| Prices | $384.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP ChIP WB IHC-P |
| Species Reactivities | Human, Mouse, Chimpanzee, Monkey, Ape, Orangutan |
| Antigen | Immunogen was a synthetic peptide, which represented a portion of human CREB binding protein encoded within exon 33 (LocusLink ID 1387)(between residues 2392 and the c-terminus (residue 2442) according to Swiss-Prot entry Q92793) |
| Description | Rabbit Polyclonal |
| Gene | CREBBP |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling KAT3A / CBP with ab10490 at 1/500 (2µg/ml). Detection: DAB.
ab10490 immunoprecipitation of CREB Binding Protein.Samples: Nuclear (NE) or cytoplasmic (S100) extract (10 mg) from HeLa cells. Antibody: ab10490 used at 20 µg/10 mg extract. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is CREB Binding Protein.
Sonicated chromatin prepared from U2OS cells was subjected to the ChIP procedure with ab10490 to CBP. Immunoprecipitated chromatin was analysed in the promoter region of c-FOS (active) and in exon 2 of MYO-D (inactive), values are % of inputs. 2–4 µg of ab10490 and 1-2x106 cells were used in each ChIP experiment. Sergei Denissov, Prof. Henk Stunnenberg lab, University Nijmengen, NL