Anti-KAT3B / p300 antibody [NM11]
| Name | Anti-KAT3B / p300 antibody [NM11] |
|---|---|
| Supplier | Abcam |
| Catalog | ab3164 |
| Prices | $401.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | NM11 |
| Applications | ICC/IF ICC/IF IHC-P IP WB FC |
| Species Reactivities | Mouse, Rat, Human, Pig, Mink, Monkey |
| Antigen | E1A affinity purified p300 protein |
| Description | Mouse Monoclonal |
| Gene | EP300 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab3164 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3164, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.
ab3164 (4µg/ml) staining KAT3B in human bladder using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of muscle nuclei.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
![Overlay histogram showing HeLa cells stained with ab3164 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3164, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/11550_KAT3B-p300-Primary-antibodies-ab3164-3.jpg)
Overlay histogram showing HeLa cells stained with ab3164 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3164, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Expression of p300 and CBP is associated with poor prognosis in small cell lung - Expression of p300 and CBP is associated with poor prognosis in small cell lung
Gao Y, Geng J, Hong X, Qi J, Teng Y, Yang Y, Qu D, Chen G. Int J Clin Exp Pathol. 2014 Jan 15;7(2):760-7. eCollection 2014.
Sublytic C5b-9 triggers glomerular mesangial cell apoptosis via XAF1 gene - Sublytic C5b-9 triggers glomerular mesangial cell apoptosis via XAF1 gene
Qiu W, Zhou J, Zhu G, Zhao D, He F, Zhang J, Lu Y, Yu T, Liu L, Wang Y. Cell Death Dis. 2014 Apr 17;5:e1176.
Regulation of RNA polymerase II activation by histone acetylation in single - Regulation of RNA polymerase II activation by histone acetylation in single
Stasevich TJ, Hayashi-Takanaka Y, Sato Y, Maehara K, Ohkawa Y, Sakata-Sogawa K, Tokunaga M, Nagase T, Nozaki N, McNally JG, Kimura H. Nature. 2014 Dec 11;516(7530):272-5.
PPARdelta deficiency disrupts hypoxia-mediated tumorigenic potential of colon - PPARdelta deficiency disrupts hypoxia-mediated tumorigenic potential of colon
Jeong E, Koo JE, Yeon SH, Kwak MK, Hwang DH, Lee JY. Mol Carcinog. 2014 Nov;53(11):926-37.
Acetylation of RNA polymerase II regulates growth-factor-induced gene - Acetylation of RNA polymerase II regulates growth-factor-induced gene
Schroder S, Herker E, Itzen F, He D, Thomas S, Gilchrist DA, Kaehlcke K, Cho S, Pollard KS, Capra JA, Schnolzer M, Cole PA, Geyer M, Bruneau BG, Adelman K, Ott M. Mol Cell. 2013 Nov 7;52(3):314-24.
In vitro phosphorylation and acetylation of the murine pocket protein Rb2/p130. - In vitro phosphorylation and acetylation of the murine pocket protein Rb2/p130.
Saeed M, Schwarze F, Loidl A, Meraner J, Lechner M, Loidl P. PLoS One. 2012;7(9):e46174.
HDAC inhibition promotes neuronal outgrowth and counteracts growth cone collapse - HDAC inhibition promotes neuronal outgrowth and counteracts growth cone collapse
Gaub P, Tedeschi A, Puttagunta R, Nguyen T, Schmandke A, Di Giovanni S. Cell Death Differ. 2010 Sep;17(9):1392-408.
Molecular basis of the interactions between the p73 N terminus and p300: effects - Molecular basis of the interactions between the p73 N terminus and p300: effects
Burge S, Teufel DP, Townsley FM, Freund SM, Bycroft M, Fersht AR. Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3142-7.
p300-mediated acetylation of the Rothmund-Thomson-syndrome gene product RECQL4 - p300-mediated acetylation of the Rothmund-Thomson-syndrome gene product RECQL4
Dietschy T, Shevelev I, Pena-Diaz J, Huhn D, Kuenzle S, Mak R, Miah MF, Hess D, Fey M, Hottiger MO, Janscak P, Stagljar I. J Cell Sci. 2009 Apr 15;122(Pt 8):1258-67.
Spatiotemporal expression of histone acetyltransferases, p300 and CBP, in - Spatiotemporal expression of histone acetyltransferases, p300 and CBP, in
Chen G, Zhu J, Lv T, Wu G, Sun H, Huang X, Tian J. J Biomed Sci. 2009 Feb 23;16:24.