Anti-KAT3B / p300 antibody - ChIP Grade
| Name | Anti-KAT3B / p300 antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab54984 |
| Prices | $395.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | IHC-P ChIP WB ICC/IF ICC/IF IP FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment: PPLQHHGQLA QPGALNPPMG YGPRMQQPSN QGQFLPQTQF PSQGMNVTNI PLAPSSGQAP VSQAQMSSSS CPVNSPIMPP GSQGSHIHCP QLPQPALHQN , corresponding to amino acids 731-831 of Human p300 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | EP300 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab54984 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54984, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/11553_ab54984-4-ab54984FC.jpg)
Overlay histogram showing HeLa cells stained with ab54984 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54984, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
p300 antibody (ab54984) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human salivary gland.
Western blot against tagged recombinant protein immunogen using ab54984 p300 antibody at 1ug/ml.
ab54984 at 10 ug/ml staining KAT3B / p300 in human Hela cells by Immunocytochemistry / Immunofluorescence.
Product References
Role of beta-catenin-regulated CCN matricellular proteins in epithelial repair - Role of beta-catenin-regulated CCN matricellular proteins in epithelial repair
Zemans RL, McClendon J, Aschner Y, Briones N, Young SK, Lau LF, Kahn M, Downey GP. Am J Physiol Lung Cell Mol Physiol. 2013 Mar 15;304(6):L415-27. doi:
High expression of p300 has an unfavorable impact on survival in resectable - High expression of p300 has an unfavorable impact on survival in resectable
Li Y, Yang HX, Luo RZ, Zhang Y, Li M, Wang X, Jia WH. Ann Thorac Surg. 2011 May;91(5):1531-8.