Anti-KAT7 / Hbo1 / MYST2 antibody
| Name | Anti-KAT7 / Hbo1 / MYST2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab70183 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ELISA ICC/IF ICC/IF IP |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide derived from an internal region of human KAT7/ Hbo1/ MYST2 |
| Description | Rabbit Polyclonal |
| Gene | KAT7 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-KAT7 / Hbo1 / MYST2 antibody (ab70183) at 1/500 dilutionLane 1 : Jurkat cell extractLane 2 : Jurkat cell extract with immunising peptide at 5 µgLysates/proteins at 5 µg per lane.
HUVEC cells with ab70183 at 1/500 dilution, with and without the immunising peptide.
IHC image of ab70183 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab70183, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![KAT7 / Hbo1 / MYST2 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to KAT7 / Hbo1 / MYST2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab70183.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 71kDa; KAT7 / Hbo1 / MYST2; non specific bands - 80kDa: We are unsure as to the identity of this extra band.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/11594_KAT7-Hbo1-MYST2-Primary-antibodies-ab70183-2.jpg)
KAT7 / Hbo1 / MYST2 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to KAT7 / Hbo1 / MYST2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab70183.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 71kDa; KAT7 / Hbo1 / MYST2; non specific bands - 80kDa: We are unsure as to the identity of this extra band.
Product References
SCF(Fbxw15) mediates histone acetyltransferase binding to origin recognition - SCF(Fbxw15) mediates histone acetyltransferase binding to origin recognition
Zou C, Chen Y, Smith RM, Snavely C, Li J, Coon TA, Chen BB, Zhao Y, Mallampalli RK. J Biol Chem. 2013 Mar 1;288(9):6306-16.
Exchange of associated factors directs a switch in HBO1 acetyltransferase histone - Exchange of associated factors directs a switch in HBO1 acetyltransferase histone
Lalonde ME, Avvakumov N, Glass KC, Joncas FH, Saksouk N, Holliday M, Paquet E, Yan K, Tong Q, Klein BJ, Tan S, Yang XJ, Kutateladze TG, Cote J. Genes Dev. 2013 Sep 15;27(18):2009-24.
An expanded Oct4 interaction network: implications for stem cell biology, - An expanded Oct4 interaction network: implications for stem cell biology,
Pardo M, Lang B, Yu L, Prosser H, Bradley A, Babu MM, Choudhary J. Cell Stem Cell. 2010 Apr 2;6(4):382-95.