All lanes : Anti-KCNC4 antibody (ab79173) at 1/500 dilutionLane 1 : extracts from COS7 cells, treated with Anisomycin (25 µg/ml, 30 mins)Lane 2 : extracts from COS7 cells, treated with Anisomycin (25 µg/ml, 30 mins) with immunizing peptide at 10 µgLysates/proteins at 20 µg per lane.
Immunohistochemical analysis of KCNC4 in paraffin embedded human brain tissue using a 1/50 dilution of ab79173, with (right) or without (left) pre treatment with the immunizing peptide.
Immunofluorescence analysis of KCNC4 in HeLa cells, using a 1/500 dilution of ab79173 with (right) or without (left) pre treatment with the immunizing peptide.