E-Cadherin (4A2) Mouse mAb

• Supplier: Cell Signaling Technology
• Catalog: 14472
• Prices: $250.00
• Sizes: 100 µl (10 western blots)
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 4A2
• Applications: WB IP IHC-P ICC/IF FC
• Species Reactivities: Human, Mouse, Rat
• Antigen: Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human E-cadherin protein.
• Description: Mouse Monoclonal
• Gene: CDH1

Product images

Western blot analysis of extracts from various cell types using E-Cadherin (4A2) Mouse mAb.

Western blot analysis of extracts from MCF7 (+) and HeLa (-) cells using E-Cadherin (4A2) Mouse mAb (upper) and Pan-Cadherin (28E12) Rabbit mAb #4073 (lower).

Immunoprecipitation of E-cadherin from MCF7 cell extracts using Mouse (G3A1) mAb IgG1 Isotype Control #5415 (lane 2) or E-Cadherin (4A2) Mouse mAb (lane 3). Lane 1 is 10% input. Western blot was performed using E-Cadherin (4A2) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded MCF7 (left) and HeLa (right) cell pellets using E-Cadherin (4A2) Mouse mAb.

Confocal immunofluorescent analysis of MCF7 (positive, left) and HeLa (negative, right) cells using E-Cadherin (4A2) Mouse mAb (green). Blue pseudocolor= DRAQ5 ® #4084 (fluorescent DNA dye).

Flow cytometric analysis of HeLa cells (blue) and MCF7 cells (green) using E-Cadherin (4A2) Mouse mAb. Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4408 was used as a secondary antibody.