Anti-KCTD12 antibody (ab176201) at 1/1000 dilution + T47D cell line lysates at 35 µg
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human brain tissue labeling KCTD12 with ab176201 at 1/50 diution, followed by peroxidase-conjugation to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of T47D cells labeling KCTD12 with ab176201 at 1/10 dilution (right histogram), compared to a negative control cell (left histogram).FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.