Anti-KCTD21 antibody [EPR12873(2)] (ab192259) at 1/1000 dilution + human fetal brain lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Anti-KCTD21 antibody [EPR12873(2)] (ab192259) at 1/1000 dilution + Caco-2 cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-KCTD21 antibody [EPR12873(2)] (ab192259) at 1/1000 dilutionLane 1 : Mouse brain lysateLane 2 : Rat brain lysateLane 3 : C6 cell lysateLane 4 : RAW264.7 cell lysateLane 5 : PC-12 cell lysateLane 6 : NIH/3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow Cytometry analysis of Caco-2 cells labeling KCTD21 using ab192259 at a 1/220 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.