Anti-KDM6B / JMJD3 antibody
| Name | Anti-KDM6B / JMJD3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab38113 |
| Prices | $382.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF ChIP |
| Species Reactivities | Human, Mouse |
| Antigen | Synthetic peptide corresponding to Human KDM6B/ JMJD3 conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Description | Rabbit Polyclonal |
| Gene | KDM6B |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab38113 stained HeLa cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab38113, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, MCF7 and HepG2 cells fixed in 100% methanol at 5ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
IHC image of KDM6B/JMJD3 staining in human liver carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38113, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product References
Ezh1 and Ezh2 differentially regulate PSD-95 gene transcription in developing - Ezh1 and Ezh2 differentially regulate PSD-95 gene transcription in developing
Henriquez B, Bustos FJ, Aguilar R, Becerra A, Simon F, Montecino M, van Zundert B. Mol Cell Neurosci. 2013 Nov;57:130-43.
p53 interaction with JMJD3 results in its nuclear distribution during mouse - p53 interaction with JMJD3 results in its nuclear distribution during mouse
Sola S, Xavier JM, Santos DM, Aranha MM, Morgado AL, Jepsen K, Rodrigues CM. PLoS One. 2011 Mar 31;6(3):e18421.
Nodal signaling recruits the histone demethylase Jmjd3 to counteract - Nodal signaling recruits the histone demethylase Jmjd3 to counteract
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A novel zinc finger protein Zfp277 mediates transcriptional repression of the - A novel zinc finger protein Zfp277 mediates transcriptional repression of the
Negishi M, Saraya A, Mochizuki S, Helin K, Koseki H, Iwama A. PLoS One. 2010 Aug 24;5(8):e12373.
Folic acid remodels chromatin on Hes1 and Neurog2 promoters during caudal neural - Folic acid remodels chromatin on Hes1 and Neurog2 promoters during caudal neural
Ichi S, Costa FF, Bischof JM, Nakazaki H, Shen YW, Boshnjaku V, Sharma S, Mania-Farnell B, McLone DG, Tomita T, Soares MB, Mayanil CS. J Biol Chem. 2010 Nov 19;285(47):36922-32.
Histone demethylase JMJD3 contributes to epigenetic control of INK4a/ARF by - Histone demethylase JMJD3 contributes to epigenetic control of INK4a/ARF by
Barradas M, Anderton E, Acosta JC, Li S, Banito A, Rodriguez-Niedenfuhr M, Maertens G, Banck M, Zhou MM, Walsh MJ, Peters G, Gil J. Genes Dev. 2009 May 15;23(10):1177-82.