Anti-Keap1 antibody
| Name | Anti-Keap1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab66620 |
| Prices | $395.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P |
| Species Reactivities | Mouse, Rat, Human, Rabbit, Horse, Guinea Pig, Bovine, Cat, Dog, Pig, Zebrafish |
| Antigen | Synthetic peptide: ETWTFVAPMK HRRSALGITV HQGRIYVLGG YDGHTFLDSV ECYDPDTDTW , corresponding to amino acids 542-591 of Human Keap1 Run BLAST with Run BLAST with |
| Blocking Peptide | Human Keap1 peptide |
| Description | Rabbit Polyclonal |
| Gene | KEAP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Keap1 with ab66620 at a concentration of 4-8µg/ml. Arrows indicate positively stained epithelial cells of the renal tubule. Magnification: 400X.
ICC/IF image of ab66620 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66620, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Lane 1 : Anti-Keap1 antibody (ab66620) at 5 µg/mlLane 2 : Anti-Keap1 antibody (ab66620) at 2.5 µg/mlLane 3 : Anti-Keap1 antibody (ab66620) at 1.25 µg/mlLane 1 : Cell lysate prepared form HepG2 cellsLane 2 : Cell lysate prepared form HepG2 cellsLane 3 : Cell lysate prepared form HepG2 cellsLysates/proteins at 25 µg/ml per lane.
All lanes : Anti-Keap1 antibody (ab66620) at 1.25 µg/mlLane 1 : HepG2 cell lysateLane 2 : HepG2 cell lysate with blocking peptide at 1 µg/mlLysates/proteins at 25 µg per lane.
Anti-Keap1 antibody (ab66620) at 1 µg/ml + Lysate prepared from human 293T cells at 10 µgSecondaryIRDye® 700DX-conjugated donkey polyclonal to rabbit IgG at 1/3000 dilutionPerformed under reducing conditions.
Product References
Ethyl gallate attenuates acute lung injury through Nrf2 signaling. - Ethyl gallate attenuates acute lung injury through Nrf2 signaling.
Mehla K, Balwani S, Agrawal A, Ghosh B. Biochimie. 2013 Dec;95(12):2404-14.
Thyroid hormone-induced cytosol-to-nuclear translocation of rat liver Nrf2 is - Thyroid hormone-induced cytosol-to-nuclear translocation of rat liver Nrf2 is
Videla LA, Cornejo P, Romanque P, Santibanez C, Castillo I, Vargas R. ScientificWorldJournal. 2012;2012:301494.